LOCUS       40924_39198        4095 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pSEVA-Plac-Turbo YFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Zhang C, Parrello D, Brown PJ, Wall JD, Hu Z.
  TITLE     A novel whole-cell biosensor of Pseudomonas aeruginosa to monitor 
            the expression of quorum sensing genes
  JOURNAL   Appl. Microbiol. Biotechnol. (2018) In press
  PUBMED    29730766
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Zhang C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4095)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2018) Department of Civil "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    282..298
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    315..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             385..1113
                     /codon_start=1
                     /label=TurboYFP
                     /note="enhanced derivative of PhiYFP"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYDFDAGSGDTS"
     terminator      1127..1221
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1353..2165
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2335..2443
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2457..3116)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(3117..3888)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(4003..4089)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt accatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      241 tcgtcttcac ctcgagaaaa tttatcaaaa agagtgttga cttgtgagcg gataacaatg
      301 atacttagat tcaattgtga gcggataaca atttcacaca tctagagtcg acctgcaggc
      361 atgcaagctt aggaggaaaa acatatgtca tccggcgcat tgttattcca tggcaagatc
      421 ccgtatgtgg tggaaatgga aggtaacgtt gacggtcaca cctttagcat tcgcgggaaa
      481 ggttacggag atgcctctgt cggtaaagtc gatgcgcagt ttatctgcac tacaggtgat
      541 gtcccagtac cgtggtctac acttgtgact accttaacgt atggtgccca gtgttttgcg
      601 aaatatggcc ctgaactgaa agacttctac aaatcgtgta tgcccgatgg ctatgtgcaa
      661 gaacggacca ttacctttga aggcgatggc aatttcaaga cccgtgcgga agtgaccttt
      721 gagaatggga gtgtgtataa ccgcgttaaa ctgaacggac agggctttaa gaaagatggc
      781 catgttctgg gcaagaatct ggagttcaac tttacgccgc attgcctgta catttggggc
      841 gatcaagcga atcatgggtt gaaaagcgcc tttaaaatct gccatgaaat tacaggtagc
      901 aaaggggatt tcatcgtagc tgaccatacc cagatgaaca cgccgattgg aggtggtcca
      961 gtccatgtac cggagtacca ccacatgtcc taccacgtga aactcagcaa agacgtgact
     1021 gaccaccgtg acaacatgtc gctgaaagaa acggttcgtg ctgttgactg tcgcaaaacc
     1081 tatgatttcg atgcaggctc aggtgatacg agttaaacta gtcttggact cctgttgata
     1141 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1201 gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa tttgtgtctc
     1261 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1321 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcagcgt gaaacgagct
     1381 gtagccgtcc gcgtctgaac agcaacatgg atgcggatct gtatggctat aaatgggcgc
     1441 gtgataacgt gggtcagagc ggcgcgacca tttatcgtct gtatggcaaa ccggatgcgc
     1501 cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa cgatgtgacc gatgaaatgg
     1561 tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac cattaaacat tttattcgca
     1621 ccccggatga tgcgtggctg ctgaccaccg cgattccggg caaaaccgcg tttcaggtgc
     1681 tggaagaata tccggatagc ggcgaaaaca ttgtggatgc gctggccgtg tttctgcgtc
     1741 gtctgcatag cattccggtg tgcaactgcc cgtttaacag cgatcgtgtg tttcgtctgg
     1801 cccaggcgca gagccgtatg aacaacggcc tggtggatgc gagcgatttt gatgatgaac
     1861 gtaacggctg gccggtggaa caggtgtgga aagaaatgca taaactgctg ccgtttagcc
     1921 cggatagcgt ggtgacccac ggcgatttta gcctggataa cctgattttc gatgaaggca
     1981 aactgattgg ctgcattgat gtgggccgtg tgggcattgc ggatcgttat caggatctgg
     2041 ccattctgtg gaactgcctg ggcgaattta gcccgagcct gcaaaaacgt ctgtttcaga
     2101 aatatggcat tgataatccg gatatgaaca aactgcaatt tcatctgatg ctggatgaat
     2161 ttttctaata attaattgga ccgcggtccg cgcgttgtcc ttttccgctg cataaccctg
     2221 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     2281 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     2341 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     2401 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccctaccgg
     2461 cgcggcagcg ttacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
     2521 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
     2581 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
     2641 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
     2701 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
     2761 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
     2821 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
     2881 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
     2941 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc
     3001 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc
     3061 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt
     3121 atctccatgc ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt
     3181 tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt
     3241 taacctacgc aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt
     3301 tttaagttgt tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc
     3361 aaagaagggc acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa
     3421 aagtggcccc tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc
     3481 gctgccccct tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc
     3541 ttcgcccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca
     3601 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca
     3661 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat
     3721 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag
     3781 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc
     3841 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg
     3901 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag
     3961 caagcccgta gggggggcgc gcccagctgt ctagggcggc ggatttgtcc tactcaggag
     4021 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     4081 ttttatttga tgcct
//
